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Vliv rostlin s různou ekonomickou strategií na půdní procesy
ŠKOPOVÁ, Kateřina
This bachelor thesis addresses plant-soil interactions through plant litter and living roots, compares the effects of plants with a competitive and conservative resource management strategy on soil and ecosystem functioning. The literature review is followed by a proposal for a scientific project aimed at increasing knowledge of the rhizosphere processes of competitive and conservative species and testing the validity of the hypothesis that competitive species affect their environment more intensively than conservative species.
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Cellulolytic fungi and their diversity on plant litter
Gálová, Diana ; Baldrian, Petr (advisor) ; Kolařík, Miroslav (referee)
Litter decomposition requires the presence of corresponding degradative enzymes, produced mainly by fungi. Forest soils show considerable spatial heterogeneity of distribution of these enzymes at diferent scales. Moreover, enzyme pruduction varies during the year, usually accompanied by the change in fungal community composition. In this work I examined if this spatial heterogeneity can be seen even at a scale of an individual leaf and whether the fungal community differs among enzyme activity hotspots and inactive parts of the leaves. Another goal was isolation of celulytic fungi from cellulose litterbags incubated on forest floor using particle filtration and dilution-to-extinction method. In a broadleaved forest dominated by oak leaves at different stages of decay were collected: senescent leaves on twigs, and leaves after 2, 10 and 22 months of decomposition. Ten leaves per season were taken for analysis of cellobiohydrolase activity over the leaf surface. Leaves were attachmed onto melted agarose plate and leaf surface was covered with low melting point agarose containing fluorescently labelled substrate. For each leaf a map of enzyme activity was created and area with the high and low enzyme activity was identified. From both sites a square of approx. 1 cm2 was cut out, DNA was extracted and fungal...
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Cellulolytic fungi and their diversity on plant litter
Gálová, Diana ; Baldrian, Petr (advisor) ; Kolařík, Miroslav (referee)
Litter decomposition requires the presence of corresponding degradative enzymes, produced mainly by fungi. Forest soils show considerable spatial heterogeneity of distribution of these enzymes at diferent scales. Moreover, enzyme pruduction varies during the year, usually accompanied by the change in fungal community composition. In this work I examined if this spatial heterogeneity can be seen even at a scale of an individual leaf and whether the fungal community differs among enzyme activity hotspots and inactive parts of the leaves. Another goal was isolation of celulytic fungi from cellulose litterbags incubated on forest floor using particle filtration and dilution-to-extinction method. In a broadleaved forest dominated by oak leaves at different stages of decay were collected: senescent leaves on twigs, and leaves after 2, 10 and 22 months of decomposition. Ten leaves per season were taken for analysis of cellobiohydrolase activity over the leaf surface. Leaves were attachmed onto melted agarose plate and leaf surface was covered with low melting point agarose containing fluorescently labelled substrate. For each leaf a map of enzyme activity was created and area with the high and low enzyme activity was identified. From both sites a square of approx. 1 cm2 was cut out, DNA was extracted and fungal...
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Does ligninolytic enzyme activity depend on phenolics content during the litter decomposition?
APPLOVÁ, Markéta
The aim of the study was to determine the influence of phenolics content and inoculation with soil extract on microbial respiration, on the phenoloxidase (PhOx), peroxidase (PerOx) and newly Mn-peroxidase (MnP) activity in two dominating litter samples (Calamagrostis villosa and Picea abies) differing in phenolics content from Plešné and Čertovo lake watersheds. At PhOx and PerOx activity, the dependence on incubation temperature with L-DOPA was estimated. PhOx and MnP activities significantly increased with higher content of hardly decomposable phenolics, but decreased with water extractable phenolics content. Inoculation with soil extract had no influence on microbial respiration, enzyme activity, nor on decomposition of phenolics. Microbial respiration was significantly higher at 10°C, but average enzyme activity was comparable at 0 and 10°C. PhOx activities had temperature optimum higher than 22°C, while PerOx activities had temperature optimum at 0 - 15°C.
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Decomposition rate of plants matter in the soils of glacial lakes in Sumava
VANĚK, Daniel
Natural processes that cause differences in rates of decomposition and mineralisation of organic plants matter in soils of Sumava mountains' glacial lakes were studied using plant litter dominant in understorey of spruce forest in watershed of two lakes, Plesne and Certovo. The aim of this study is to determine the effect plant liter chemistry on decomposition and mineralisation rate. We hypothetize that chemistry of liter of understorey vegetation significantly affect N mineralisation / immobilisation in siols. During four month laboratory experiment were studied the effect of litter quality characteristics on C mineralization rates and N release. Spruce needles (Picea abies) and leaves of four dominant species (Avenella flexuosa, Calamagrostis villosa, Vaccinium myrthillus and Athyrium alpestre) of understorey vegetation were collected in autumn 2006. Litter was incubated at 0 and 10 °C. CO2 release was regularly measured each 14 days. Amounts of phenolics, available phosphorus, total and extractable C and N, release of NH4+ and NO3- were measured at the beginning, after one and four months of incubation.
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